Gene-targeting technologies using sequence specific nucleases have been widely adopted to induce genetic modifications in plant genome, of which generating targeted point mutation for gain-of-function phenotype is of great value. Although tremendous efforts have been made in developing gene insertion or replacement approaches by harnessing DNA repair pathways through either homologous recombination or non-homologous end joining mechanisms, introducing specific DNA sequence changes into genome at the desired site remains challenging. Recently, researchers at State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection of CAAS have reported A CRISPR/Cas9 toolkit for efficient targeted base editing to induce genetic variations in rice.